In its early days, the assay delivered information in a strict binary of ‘yes’ or ‘no’ – protein present or absent. In recent years, however, improved western blot technology and technique have allowed an exciting quantitative element to emerge. Researchers can now reliably measure relative changes in protein expression and abundance.īut with this shift towards quantitative understanding comes a movement by major journals, funding agencies, and professional scientific societies to uphold the highest standards of data integrity for publication. Their updated guidelines for immunoblotting images come alongside guardrails against data manipulation, intentional or inadvertent, in a growing age of generative AI and accessible image editing software. Quantitative Western Blotting Requires Normalization So, what does a “publication-ready” western blot mean today to the top journals in the field, and what tips, tools, and products exist to help researchers get there? This comprehensive guide will help steer you through the process. With a quantitative western blot, researchers can gain valuable insight into relative protein expression. Quantitative western blotting requires careful interpretation, particularly around non-uniformities. Variability in western blotting occurs most often with unequal protein concentrations, inconsistent sample loading onto the gel, and irregularities during transfer – all normal, human realities of the process. Normalization accounts for those realities by distinguishing experimental variability from true biological changes in protein expression. Skillful normalization ultimately encourages accuracy and reproducibility in the literature. Right now, methods for immunoblotting normalization and quantitation across the field vary more than many experts would like.Įditorial staff of the field-leading publication Journal of Biological Chemistry named acceptable presentation and quantitation of western blots as one of three “ major gaps in overall data reporting” among submissions. Their recently issued revised guidelines for blot reporting reflect a wider push towards total protein normalization (TPN) in lieu of another popular normalization strategy using housekeeping proteins (HKP). Let’s talk through some of the downsides of housekeeping protein protocols and advantages of total protein normalization. Housekeeping Proteins (HKP) Are Falling Out of Favor with Journalsįor decades, researchers have used housekeeping proteins like GAPDH, β-tubulin, β-actin, or cyclophilin B as internal loading controls for normalization. In this approach, changes in a sample’s target protein level can be expressed as a ratio to the chosen HKP. HKP is still the most-used method of western blotting normalization today. The problem with this protocol is that it assumes consistent HKP expression across experiments, and more and more studies have now confirmed that this just isn’t the case. ![]() HKP expression is more variable than constant, changing with cell type and developmental stage tissue age, type, and pathology post-transcriptional regulation experimental conditions and more (Table 1).
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I just thought it would be so easy to do this using USB but here I am about ready to pull my hair out, I'm very frustrated with this.ītw I've got a Pixel 6a smartphone and it came with a USB adapter that plugs into the USB-C port on the phone, that way I can hook up a regular USB cable to the phone. I guess if I'm told "Hey don't bother with USB, just use KDE." Then I guess I'll consider doing that. Please I just need experts to tell me what to do here? What do i do? But it looks like transferring files via USB isn't going to be as easy as I thought it'd be on Linux. I don't know, should I just go with KDE? But is KDE 100% secure? Whereas I know a USB wire is totally secure. So like I said, it seems to me basically everyone just uses KDE Connect, it seems hardly anyone even bothers with USB anymore. ![]() I mean I thought it was going to be so easy, I thought I'd just plug my phone into my PC and transfer a few files, I didn't think it'd be this hard. This turned up on Google search though, this article tells you how to transfer files via USB on Linux but how accurate is it? I went and googled "how to connect android smartphone to computer through usb linux" and hardly any search results turned up, and the ones that did turn up are out of date (it seems to me the vast majority of Linux users just use KDE Connect app, it seems hardly anyone bothers with USB anymore) but I really don't want to transfer files wirelessly I'd much rather do this through a wire. So what is the definitive way to plug my phone into my PC via USB and transfer files from phone to PC or vice versa? So yeah it seems to me most people don't bother with USB anymore and just connect wirelessly using KDE Connect app but honestly I'd rather just do it wired, wired is the most secure way to do it in my opinion, I just want to plug my phone into the PC via USB and transfer files. ![]() ![]() Inuyasha - Español Latino (versión Televix)ģ Inuyasha Quad Movie Collection InuYasha (BDRip 960x720 HEVC AAC)ġ Inuyasha All Episodes 1-167 ġ Inuyasha (001-098) Inuyasha - 001-167 Dual Audio ġ InuYasha 2000 720p BluRay x264 AAC-Shiniori ![]() Inuyasha_TV+Finale+OVA+Film+CD+Manga+Other dub jpn,chn,eng sub chs () Basilisk, BlackCat, Last Exile, Solty Rei, Glass Fleet, Inuyasha S1.etc ġ4 Inuyasha S2 (028-054) (Dual Audio)ģ8 Inuyasha (BD, 720p) (US BD Source)ĩ2 Inuyasha (BD, 720p) (Spanish BD source) They do however make the best chips and roasted veg. Rotary types are not usually good for recipes where the food is breaded, glazed, likely to break up or in a tray/container. This is the type I used for years – the most common is a Tefal Actifry. Round air fryers with a paddle in the centre that rotates to move the food. I’ve since bought this version to use for some recipes where my smaller model was just too small. The unit is no larger than the 3.8 litre model on the outside but does have a larger internal basket capacity. This is the Cosori 4.7L (CAF-L501) with a 4.7 litre basket. I’ve also recommended to several friends and family the model with a slightly larger basket. The square(ish) shaped basket is 21cm x 21cm and 24cm on the diagonal. It isn’t an overly expensive or fancy model. ![]() I mostly use a Cosori Lite 3.8L (CAF-LI401S) with a 3.8 litre capacity. This is the type that you’ll see in the images in this recipe. Some larger models have two drawers so different items can be cooked at different temperatures or for different times. Food isn’t usually visible whilst cooking. A boxy type unit has a pull out drawer/basket. These are what I would consider to be the most common, especially in the UK. I consider there to be 4 main types: Basket Air Fryers Nope! The term air fryer is now used very broadly. For slightly thicker chops you will need to increase the cooking time. They range from 180 to 190g (6.3 to 6.7oz) and are around 1cm (3/8″) thick.įor even thinner chops you will need to reduce the cooking time. The thin chops I’ve used were a budget supermarket buy. The air fryer is a brilliant way to get that fat lovely and crispy. The width of the fat can vary so select your chops to your preference. Do make sure that you rinse the chops and dry them thoroughly to get rid of any of these pieces.Ī good chop has a layer of fat on the side opposite the bone. The cheaper chops can be poorly packed and not have been washed properly to get rid of any little shards of bone which have pinged off during the cutting process. I do find that it is very common for thin chops especially to only come with a very small piece of bone attached. The meat cooked next to the bone is extra delicious. Take the bone off and you have loin steaks which are also unsurprisingly called boneless pork chops. Ingredients to Air Fry Pork Loin Steaks Pork ChopsĪ true pork chop includes the bone and the chop is cooked with the bone still attached. ![]() ![]() Interactive Web-Based Tiles: Livetiles breaks the norm by introducing customizable tile formats that bring dynamic web content directly to your desktop.Think customizable widgets powered by online tools and web apps.Ĭonvert just about any web destination into a live tile showing content that stays up-to-date on your desktop from to-do lists to stock tickers to remote system monitors to weather forecasts and everything in between. Most live wallpapers focus on background animation but Livetiles takes a novel approach by bringing web content to life in customizable tile formats on your desktop. Smooth Performance: Emphasizes seamless performance to prevent lags or disruptions while displaying animations.Focus on Aesthetics: Prioritizes visual allure and elegance over complexity, ensuring a visually captivating desktop experience.Mesmerizing Animated Wallpapers: Offers a diverse range of animated wallpapers spanning colorful abstract designs to futuristic space scenes.By mixing and matching elements from different skins, you can create extensively customized live wallpapers. You have fine-grained control over sizing, animations, effects, colors, assets, and more. You’ll find everything from audio visualizers that dance to your music to CPU usage sparks to abstract 3D landscapes.Īs a modular desktop suite, Rainmeter wallpapers are crafted as standalone skins that you can install and tweak to perfection. While best known as a customizable desktop suite, Rainmeter offers some standout animated and interactive live wallpapers courtesy of its vibrant user community. Customizable Settings: Allows users to tweak settings for wallpaper transitions and updates.Minimal System Impact: Designed to run seamlessly in the background without consuming excess resources.Location-Based Sunlight Data: Adjusts wallpapers based on your geographical location and time zone.Integration with macOS Feature: Similar to macOS Mojave’s dynamic wallpaper feature for Windows.You can customize the wallpapers to your liking with controls over playback, sound, speed, colors, transitions, and more. The wallpapers go beyond just animation with many featuring sound effects and even music support. Both 2D and 3D wallpapers are available with a focus on quality over quantity. It offers thousands of free and paid premium options crafted by a vibrant artist community. ![]() sets the gold standard when it comes to animated wallpapers for Windows. In this article, we showcase the top 15 best picks along with their key features and a deeper look at what each offers. There are many great free live wallpaper apps for Windows 10 and 11 to choose from. They add style and personality that static wallpapers cannot achieve. Live wallpapers can make your Windows desktop stand out with animated backgrounds and visual effects. Here you go! 15 Best Free Live Wallpaper Apps for Windows From serene landscapes to dynamic animations, these top 15 free Windows apps for live wallpaper offer a gateway to personalized and immersive desktop aesthetics.Įlevate your desktop beyond static backgrounds and infuse it with vibrant life using these innovative and easy-to-use live wallpaper apps for PCs. Transforming your desktop experience into a captivating visual journey is easier than ever with a plethora of live wallpaper apps available for Windows 10 and 11. Implement any new strategies and procedures you’ve come up with. Analyze their current processes and assess changes. Learn and understand everything you can about a company from their processes, customers, products, the competition to procedures.Įvaluate and put your plan into action. Generally, the 30-60-90-day sales plan can be broken down into 3 sections: ![]() The plan includes milestones they’d need to achieve at the 30th, 60th, and 90th day of their ramp-up. This is designed to help a new salesperson or sales manager get up to speed quickly in their first quarter on the job. We’ll go over a few sales plan examples to get you started in the right direction. ![]() If you’re like most people, it’s the annual sales plan or weekly sales plan - broad strategic and tactical documents mapping out the plan for everything sales-related.īut there are as many different types of sales plans as there are needs for a sales plan. What usually comes to mind when you think about sales plans? Sales Plan Examples: There’s No One Right Way This section lays out performance metrics to track the systems and processes that help monitor these metrics. Tasks include prospecting activities, meeting appointments, and product demos/presentations. This section assigns tasks, activities, and responsibilities to different teams and individuals. This section provides a summary and describes the current state of all production inputs (human resources, tech software, specialized sales team, etc.,) required to process and close sales details. Team Capabilities, Resources, and Upgrades The document should describe new segments of the addressable market when they arise.ħ. This section cites all the potential revenue-generating, omnichannel opportunities available for the brand, such as the following: This section recommends the best selling techniques, communication sequences, and playbooks for the specific company. This section provides a summary of the market trends that have a high likelihood of influencing sales performance.ĥ. The overarching goal is to optimize the sales plan by adopting inputs and techniques that work. This section presents a recap of the prior period’s performance, identifying mistakes as well as decisive actions that led to a positive outcome. Classifying revenue figures based on different categories (such as line and territory) helps clarify the document. This section clearly establishes revenue targets and may include associated business goals (e.g., optimize lifecycle value through customer success programs, etc). It also states the specific period and other parameters covered by the plan. This section gives a short summary of the document, focusing on goals and the strategies to achieve them. Executive Summary and Scope of The Sales Plan Specifically, it covers 9 pieces of strategic information.ġ. The resources and activities required to carry out those strategiesĪ sales plan covers a lot of important aspects of business growth: revenue goals, selling methods and metrics, target customers, current sales force capabilities, and more.Specific revenue and performance goals for a given period. ![]() A sales plan makes it possible for everyone on the sales team to see the big picture, share the same overall objectives, and work the same plan to achieve them. Sales Plan Examples: There’s No One Right WayĪ sales plan is a strategy document that lays out a company’s plan for improving sales results in a specified time period.Keep reading for tips and a template to quickly and confidently create a strategic sales plan for your business. The question, of course, is how to create a sales plan that actually impacts sales. ![]() And for sales success, nothing beats a strategic sales plan.ĭesigned specifically to help your sales team drive more sales, a sales plan can show you where you’re at, where you want to be, and even more important, how to get there. I'm running the controller on a 2012 MBPr 13". It works very easily by allowing you to bind key strokes to any of the buttons or joysticks in the controller - not a single problem. Mavericks is fully supported in the latest release. ![]() ![]() I set this up very quickly without any problems to play Borderlands 2 (Steam edition) using a great shareware application called USB Overdrive. I could not find a single article on how to get this to work with OSX so wanted to post this to help out anyone else in the same position. It also has a pseudo XBox 360 controller layout for the left joystick / joypad which have been swapped over.Īnyhow, if anyone does purchase one of these to use for gaming on OSX, rest assured you have not wasted your money. It’s becoming a bit frustrating having to remember which controller is the backwards one and it makes it tricky to help my 6yo navigate minecraft. However, this is a very nice controller and great value at £18 delivered from Amazon. Somehow, one of my kids managed to swap the L1/L2 and R1/R2 buttons on ONE of the controllers. It should automatically download all of the other files it needs, so just follow all the prompts. In this quick and easy tutorial, Ill show you how to connect a DualShock 3 controller to a PC running Windows 10.SCP Drivers Download. If your computer doesnt have built-in Bluetooth support, plug in your wireless Bluetooth dongle. ![]() I naively bought this 3rd party controller thinking it would be Bluetooth like an offical Sony PS3 controller only to learn that due to licencing restrictions, only Sony is allowed to make Bluetooth controllers for Playstation. How do I use my Afterglow ps3 controller on PC Plug the DualShock 3 into your PC via a mini-USB cable. If all of your details are correct you will be taken to the next page. ![]() When you have finished entering your details, click Add Account. Personally, I choose not to tick this box as I want to see the emails in my inbox. If you choose to archive incoming messages then they won’t appear in your inbox. If you choose to label incoming messages then there will just be a little label next to each email in your inbox that has come from this address. Make sure you tick the box next to Always use a secure connection (SSL) when retrieving mail. This is what it will look like once you add your details to the form: Your POP Server is the incoming server and the Port is the POP3 Port. Your username is usually just your email address, and your password is the password you created when you set up this email account. To find these details, you will need to look back at the tab where you have your email configuration details. ![]() On the next page, we need to add in some of our email account details. Next, you will need to choose whether you want to link accounts with Gmailify, or whether you want to import emails from another account.Īs you can see in the image below, I’m unable to select Gmailify as its not available with my provider, but that doesn’t matter because we’re going to choose the second option instead: Import emails from my other account (POP3). Click the Add a mail account link next to this.Ī small yellow pop up window will appear.įirstly, enter the email address associated with the account that you want to add to your Gmail account. On the Settings page, click on the Accounts and Import tab, look for the Check mail from other accounts option. Once you have signed in to Gmail or created a new account, click on the cog button and click Settings. You don’t have to use this new email address if you don’t want you can just use this account specifically for your cPanel email. If you do decide to set up a new Gmail account specifically to receive and send emails using your new email address, you will need to set up a Gmail account with a brand new Gmail email address. Now you need to decide whether you want to connect your cPanel email account to an existing Gmail account that you own, or whether you’d like to set up a new Gmail account specifically for this email account. The details here are going to be very important in the for the rest of this process, so make sure you keep this page open in a tab for future reference. For this tutorial, we will be focusing on the settings in the blue box as SSL is more secure. In the blue box you’ll see the secure SSL/TLS settings, and in the yellow box, you will see the Non-SSL settings. This will take you to a new page, and on this page scroll down until you find a section called Mail Client Manual Settings. To do this, head back to the Email Account tab and click on Connect Devices then click Set Up Mail Client. I’m sure you’ll realise why I dislike these email clients! 2 | Find your email configuration detailsĪfter setting up your email account you will need to locate your email configuration details. If you click on one of these options you will be taken to your inbox. On the next page, you will see the webmail applications that come with cPanel. If you want to view your email account, click on Access Webmail. Here you will see the email account you’ve just set up. ![]() And that’s it you have a new email account! Once you’ve filled in this form, click Create Account. You will need to fill this in with your desired email address, password and mailbox quota (i.e. To create a new email account, click on the Add Email Account tab. On this page you will see a number of tabs at the top of the page. It looks like this:Ĭlick on this icon to be taken to the Email Accounts page. If you don’t already have an email account set up in cPanel, log in to your cPanel account and look for the Email Accounts icon under the Email heading. If you already have one set up that you want to connect to Gmail, great! Ignore this step and proceed to step 2. In this post, I’ll show you how to connect your cPanel email account to Gmail.įirst things first, you will need to set up an email account in cPanel. But as I say, they’re pretty awful! Their designs are very old school, and they’re not as easy to use as other email clients, such as Gmail and Outlook.īut the good news is it is possible to connect your cPanel email account to Gmail so that you can send and receive email via Gmail. So for example, my email address is, and I set this up through cPanel.ĬPanel also comes with a number of email clients that you can use to manage your email Horde, Roundcube and SquirrelMail. If you read my post about cPanel you will know that you can create new email accounts in cPanel with email addresses that use your domain name. ![]() The email clients that come with cPanel are pretty awful, in my opinion. ![]() Is being completely open to the Spirit of God and recognizing our dependence on God for all that we have and are.Is Perfect submission to our Heavenly Father.He said that having a broken heart and a contrite spirit: It all makes sense now! This new definition also made clear the things I had been studying in a talk entitled “ Broken Heart and A Contrite Spirit by Bruce D. Neither did they receive any unto baptism save they came forth with a broken submissive, trained heart and a contrite repentant, penitent spirit, and witnessed unto the church that they truly repented of all their sins. Now substitute the new found meanings in the scripture for the original words. We have a desire to repent quickly when we have done something wrong.īoth of these definitions seemed to fit so well with the scripture I quoted above from Moroni. ![]() If we have a submissive, tamed, trained heart then we also should have a repentant, penitent spirit. This fit perfectly with having a broken heart. So, to me, contrite means being remorseful when we have done somethings wrong, we feel truly sorry and we have a desire to repent. Filled with a sense of guilt and the desire for atonement penitent. A horse can finally fulfill its purpose once it is controlled and doing the bidding of his master, and only when we are submissive to our Savior can we do His will and fulfill the purposes He has for us. He expects us to come with a submissive, tamed heart! Yes! A heart that is trained or broken like we train or break a horse. With that one definition it all made sense! God doesn’t expect us to come to him with a broken, out of order heart. And then I got to the definition that made everything clear!īroken: “Tamed, trained, or reduced to submission: The horse was broken to the saddle.” While I know that we can bring our weakened, fragmented, not working parts to Christ and He will heal us, it didn’t seem right that a heart that is out of order and not working properly would be a requirement of baptism. These definitions still didn’t sound right. I found, as I had expected, definitions like: reduced to fragments fragmented, ruptured torn, not functioning properly, out of working order, fragmentary or incomplete. We’ll start with the word broken because it is listed first. I thought I knew what the word broken meant, and I knew I didn’t have a clear understanding of the word contrite. I decided to look up the words broken and contrite in the dictionary, because that’s almost always where I start. I was picturing someone being in the depths of despair over the loss of someone they loved, but that doesn’t sound like the type of broken heart that was expected before people could be baptized in Moroni 6:2 when it says: “Neither did they receive any unto baptism save they came forth with a broken heart and a contrite spirit, and witnessed unto the church that they truly repented of all their sins.” We know that children as young as 8 years old can be baptized so having someone break your heart and leave your heart shattered in pieces doesn’t seem consistent with a requirement to be baptized, nor does it seem to go hand in hand with repentance. To me, up to this point, having a broken heart meant that someone you deeply loved had done something to hurt you and then your heart was broken or shattered. I thought about what having a broken heart and contrite spirit means. The topic was: “Having a Broken Heart and a Contrite Spirit.” The Spirit told me to stop, and to take time to study and learn some more about the topic. I was skimming over my scripture journal a few days ago, reviewing some of the things the Lord has taught me in the last few months, and I came to a topic that I felt like I still didn’t understand. What Does It Mean to Have a Broken Heart and a Contrite Spirit? ![]() It’s a way for us to keep the site running and provide you with valuable content. This means if you click on them and make a purchase, we may earn a small commission at no additional cost to you. Just a heads up, some of the links on ONE Esports are affiliate links. GET YOURS TODAY: Valorant US$10 Gift Card Bleed Esports’ Valorant roster Credit: Nigel Zalamea/ONE Esports The best initiators on Icebox to dominate the mapīleed management has emphasized their commitment to closely monitoring the performance of all players to ensure that the final roster is well-suited and prepared for the challenges of the upcoming VCT Pacific league. How your Valorant ranked MMR is calculated, according to Riot’s senior dev The best controllers on Icebox to block enemy sightlines Where to place your smokes on Valorant map Lotus His consistent performance at the tier-two level has earned him a well-deserved opportunity to compete professionally, making him a promising prospect for the team. Retla is a familiar face within the Bleed organization, having competed under their banner in 2022. ![]() Credit: Riot Games, ONE Esportsħ weird Valorant crosshair codes that’ll crack you up To create a circle crosshair in VALORANT, use the following inner line settings: Outlines: Off. While playing under the DRX banner, Zest was part of the Korean squad that qualified for all Valorant international LANs in 20 seasons. How to get free Masters Madrid drops: Add new title, player card to your collection He was the initiator main of the team when Vision Strikers ran their historic 104-game win streak in 2021. Zest, a seasoned veteran in the Valorant scene, boasts an impressive track record, having been part of the dominant Vision Strikes and DRX teams. The decision to bring in Zest and Retla comes after their debut performance at the VCT Kickoff tournament, where Bleed fell short against T1 and Global Esports.Īs a result, Bleed moved crazyguy to the team’s bench, while Egoist was released from the roster. But color codes also allow you to change the color of your crosshairs effortlessly. We’ve already covered how you can copy a crosshair, whether it’s an opponent’s or your favorite esports pro or content creator’s, and even create big and quirky crosshairs. Bleed Esports signs former DRX in-game leader Zest and newcomer Retla Credit: Bleed VALORANT crosshairs come in all shapes and sizes. The changes came after the team’s VCT Pacific Kickoff run, where they finished 10th-11th place. Here are all the Valorant Lotus map guides you’ll need ![]() On March 8, the Singaporean esports organization added former DRX star Kim “Zest” Ki-seok and former Bleed player Jorell “Retla” Teo to its active roster, replacing Ngo “crazyguy” Cong Anh and Javier “Egoist” Chua. Bleed Esports has made significant changes to their Valorant roster early into the 2024 VCT season. |
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